General Cell Based Assays
Eurofins Discoverx InCELL Hunter eXpress CLK1 Kinase Binding Assay, 2pl Kit. (96-0059E1CP0M)
The InCELL Hunter eXpress CLK1 Kinase Binding Assay provides an easy-to-use cell based assay to study small molecule drug candidates that engage with kinases. This kit assesses compound-engagement-based stabilization of CLK1 via detection of CLK1 levels. This product uses EFC technology. The cell line included overexpresses CLK1 target protein fused to ProLabel. In the absence of compound that binds and stabilizes the target, this protein is degraded and no EFC signal is produced. When compound engages and stabilizes the target, EA contained in the detection reagent produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
American Research Products Inc BoVine lactoperoXidase LPO E
BoVine lactoperoXidase (LPO) ELISA kit from CUSABIO Detection Range 1 56 mU/mL-100 mU/mL Sensitivity 1 56 mU/mL Sample breast milk Method Competitive
Eurofins Discoverx PathHunter eXpress NPSR1b U2OS β-Arrestin GPCR Assay, 2-Plate Kit. (93-0822E3CP0M)
The PathHunter NPSR1b eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. neuropeptide S) based activation of NPSR1b GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged NPSR1b and EA-tagged β-Arrestin-2. Activation of NPSR1b stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress MC3R CHO-K1 GPCR Assay, 2-Plate Kit. (95-0045E2CP2M)
The cAMP Hunter MC3R eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. α-melanocyte stimulating hormone) based activation of MC3R GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type MC3R, a naturally Gs coupled GPCR. Upon activation of MC3R, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress HCRTR1 Total GPCR Internalization Assay, 10pl Kit. (93-0729E3CP0L)
The PathHunter HCRTR1 Total GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of HCRTR1 via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged HCRTR1 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the HCRTR1 receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cayman Chemical DUB ActIvIty asy KIt 96Wel
Cayman’s DUB Activity Assay Kit facilitates the rapid, robust measurement of deubiquitinating enzyme activity in vitro. The kit utilizes a high purity, fluorogenic substrate (ubiquitin-AMC) together with suitable calibration standards and controls for the accurate and sensitive assessment of DUB activity. Continuous kinetic or end-point assays can be performed in a 96-well plate format for multi-sample analysis. EAch kit contains sufficient material for one full 96-well plate assay set-up to be run. Ubiquitin-AMC is not a suitable substrate for all DUBs. Compatibility must be determined by the end user.
Cayman Chemical Glycogen FluorometrIc Detecto
A GnRH receptor antagonist (Kis 6 2.2 and 3.8 nM for the human mouse and rat receptors respectively) selective for the GnRH receptor over a panel of 43 receptors ion channels and enzymes at 1 UM prevents GnRH-induced increases in inositol phosphate production in HEK293 cells expressing the human GnRH receptor (KB 25 nM) oral or intravenous administration decreases plasma LH levels in castrated rats inhibits GnRH-A-induced increases in plasma LH and testosterone levels in non-castrated rats at 20 mg/kg
Revvity Health Sciences Inc Phospho-Insulin Receptor β (Tyr1150/1151) Terbium Surefire Ultra Detection Kit, 10,000 Assay Points
Phospho-Insulin Receptor β (Tyr1150/1151) Terbium Surefire Ultra Detection Kit, 10,000 Assay Points
Abcam HDAC substrate, colorimetric (Boc-Lys(Ac)-pNA), 100MG
MW 408.45 g/mol, Purity >98%. Colorimetric substrate for histone deacetylases (HDAC). For use in HDAC activity assays.
The product is subject to the following: Abcam Restricted Use Statement
Ethos Biosciences Ellman Thiol oxidative stress 1/EA
The Ethos Biosciences DTNB (Ellman s) Thiol Oxidative Stress Assay Kit is designed to measure free thiol groups that arise due to oxidative stress which is an underlying component of acute and chronic kidney disease Our kit uses Ellman s Reagent otherwise known as DTNB or 5-5 -dithiobis [2-nitrobenzoic acid] which reacts with the disulfide bond in a sample to produce 2-nitro-5-thiobenzoic acid Under conditions of oxidative stress free thiols decrease and disulfides increase Determination of the free thiol concentration reflects the ability to detoxify lipid (and other) peroxides Aliquots of standard or sample are reacted with rehydrated Ellman s reagent (DTNB) in microtiter wells Our DTNB (Ellman s) Thiol Oxidative Stress Assay kit contains all of the components and reagents you need to conduct a 96 well plate chemical assay for thiol groups of any species in up to 40 duplicate urine or cell free liquid samples (plasma serum cell culture supernatant etc )
Eurofins Discoverx PathHunter eXpress mGLP1R CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0478E2MCP0M)
The PathHunter mGLP1R eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. exendin-4) based activation of mGLP1R GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mGLP1R and EA-tagged β-Arrestin-2. Activation of mGLP1R stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cayman Chemical LDL Uptake CellBased Assay Ki
LDL uptake and its regulation are important therapeutic targets for atherosclerosis and related diseases. Cayman Chemical’s LDL Uptake Cell-Based Assay employs a preparation of human LDL conjugated to Dylight™ 550 as a fluorescent probe for detection of LDL uptake into cultured cells. A LDL receptor-specific antibody and a Dylight™ 488-conjugated secondary antibody are included in the kit for identifying the distribution of LDL receptors. The kit provides a convenient tool for studying LDL uptake and its regulation at the cellular level.
Cayman Chemical GlutathIon asy KIt 96Wel
Cayman’s GSH Assay utilizes a carefully optimized enzymatic recycling method for the quantification of GSH in a 96-well microplate format. It measures both GSH and GSSG to reflect total glutathione in a sample. The kit can also be used to measure only GSSG by following an alternative protocol. The GSH Assay can be used for plasma, tissue samples, and cultured cells with minimal sample processing.
Abcam Adenosine 5'-monophosphate (AMP) disodium he x ahydrate, AMPK activator, 25G
MW 391.19 g/mol. Nucleotide involved in the reactions of cellular energy transfers. Acts as an activator of AMP-activated protein kinase (AMPK). Used as a substrate for enzymes such as AMP-thymidine kinase, AMP deaminase and 5'-nucleotidase.
The product is subject to the following: Abcam Restricted Use Statement